期刊名称：PROTEIN EXPRESSION AND PURIFICATION
 期刊简介(About the journal)
投稿须知(Instructions to Authors)
编辑部信息(Editorial Board)
About the journal
Protein Expression and Purification
The power of modern molecular genetics to provide large quantities of proteins that were previously difficult to obtain has sparked an explosion of interest in both practical and theoretical aspects of protein purification.
Protein Expression and Purification is dedicated to providing a forum for information about protein isolation based on conventional fractionation as well as techniques employing various molecular biological procedures to increase protein expression.
The following types of articles are published:
Original articles reporting novel or significantly improved isolations of highly purified proteins
Procedures for expressing and isolating proteins from genetically engineered sources
Novel or improved molecular biological methods for overexpression of specific proteins
Review articles that describe and evaluate important approaches to the expression and purification of proteins
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Abstracting / Indexing
- Biological Abstracts
- Chemical Abstracts
- Current Contents/Life Sciences
- Food Science and Technology Abstracts
- Index Medicus
- Research Alert
- Science Citation Index
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Instructions to Authors
Protein Expression and Purification is an international journal designed to provide biochemists, molecular biologists, and other investigators with a forum for presenting significant advances in protein isolation. The journal publishes original articles on novel or improved isolations of specific proteins from conventional and genetically engineered sources. Reports of new or improved methodologies for separation of proteins or for their overexpression are also within the scope of the journal. Review articles describing and evaluating important advances in the expression and purification of proteins will be featured. Such reviews are generally invited; interested authors should contact the Editor-in-Chief. Topics for original articles include but are not limited to:
The preparation of natural and recombinant proteins:
- Achievement of a high level of purity
- Expression of active recombinant proteins
- Production of mutant proteins
- High-throughput purification
- Improved or novel fractionation
Techniques for protein expression:
- Control of stability, solubility, and activity
- Gene constructions designed to facilitate purification
- Vectors and hosts
- High-throughput expression
Submission of Manuscripts. Manuscripts should be submitted using the Elsevier electronic submission tool. To submit your paper and associated artwork, please go to http://authors.elsevier.com/pub/622935/. A PDF is generated and the reviewing process is carried out using that PDF. All correspondence between editor and author is performed by e-mail, and paper copies are not required at the original submission stage.
- Include an abstract of 250 words or less; do not include references in the abstract.
- Include a table summarizing purification; see table instructions for specific format.
- Use the reference style shown below; note that titles are included. Unpublished papers can be cited as references only if they are in press.
- Optional: Indicate members of the Editorial Board or other independent established investigators that have special expertise to serve as reviewers of the manuscript.
Protein Expression and Purification
Editorial Office
525 B Street, Suite 1900
San Diego, CA 92101-4495, USA
Telephone: (619) 699-6813
Fax: (619) 699-6700
E-mail: pep@elsevier.com
There are no submission fees or page charges except for color figures as noted below.
Manuscripts are accepted for review with the understanding that the same work has neither been published nor is under consideration elsewhere, and that its submission for publication has been approved by all of the authors and by the institution where the work was carried out; further, that any person cited as a source of personal communications has approved such citation. Written authorization may be required at the editors' discretion. Articles and any other material published in Protein Expression and Purification represent the opinions of the authors and should not be construed to reflect the opinions of the Editor(s) or the Publisher.
Upon acceptance of an article, authors will be asked to transfer copyright (for more information on copyright, see http://authors.elsevier.com). This transfer will ensure the widest possible dissemination of information. A letter will be sent to the corresponding author confirming receipt of the manuscript. A form facilitating transfer of copyright will be provided after acceptance.
If material from other copyrighted works is included, the author(s) must obtain written permission from the copyright owners and credit the source(s) in the article. Elsevier has preprinted forms for use by authors in these cases: contact Elsevier Global Rights Department, P.O. Box 800, Oxford OX5 1DX, UK; phone: (+44) 1865 843830, fax: (+44) 1865 853333, e-mail: permissions@elsevier.com.
Preparation of Manuscript. Prepare word-processed manuscripts double-spaced throughout, including abstract, references, acknowledgments, tables, and figure legends. Number pages consecutively. Page 1 should contain the article title, authors' names (preferably including one full forename for each author), and complete affiliations. The first page should also include the name, mailing address, telephone, e-mail address, telex, and/or fax numbers of the author to whom correspondence and proofs should be sent. At the bottom of page 1 place any footnotes to the title. Page 2 of the manuscript should contain an abstract summarizing the accomplishments of the study and their significance in a single paragraph of 250 words or less.
Text. The suggested organization of an article is abstract, introductory statement, materials and methods, results, discussion, acknowledgments, and references. Some of these sections may be combined if the presentation is thereby made clearer or more effective. In all cases an abstract is required.
Protein isolation and expression procedures should be described in sufficient detail to allow the procedures to be used without extensive reference to previously published studies. Well-established procedures such as methods of protein determination and general techniques of vector construction should be cited only by literature reference, but the procedures for determining enzyme activity or for specifically quantitating nonenzymatic proteins (e.g., immunoreactivity or densitometric quantitation of protein gels) should be described in sufficient detail to allow the methods to be applied without reference to previous descriptions. For assay methods that have been reported previously, the description should be as brief as is consistent with direct application of the method; the original report of the method should be cited. For novel assay procedures, the experimental description should be complete and include evidence of specificity, sensitivity, and linearity of response with time and amount of protein.
Vector construction should be diagrammed adequately showing relevant marker genes, regulatory elements, insert and vector sizes, and key restriction enzyme sites. If indicated restriction enzyme sites are not unique in the construct, this fact must be stated. Information regarding the construction of novel vectors or recombinant molecules should be provided in sufficient detail to allow the work to be evaluated and applied. Where appropriate, DNA sequences should be reported. Previously unpublished gene or cDNA sequences must be submitted to a public data base (e.g., GenBank/ EMBL Data Bank) prior to publication; accession numbers will be included in the published paper as a footnote.
GenBank/DNA sequence linking. Authors wishing to enable other scientists to use the accession numbers cited in their papers via links to these sources should type this information in the following manner:
For each and every accession number cited in an article, authors should type the accession number in bold, underlined text. Letters in the accession number should always be capitalized (see example below). This combination of letters and format will enable Elsevier's typesetters to recognize the relevant texts as accession numbers and add the required link to GenBank's sequences.
Example: GenBank accession nos. AI631510, AI631511, AI632198, and BF223228), a B-cell tumor from a chronic lymphatic leukemia (GenBank accession no. BE675048), and a T-cell lymphoma (GenBank accession no. AA361117).
Authors are encouraged to check accession numbers used very carefully. An error in a letter or number can result in a dead link. In the final version of the printed article, the accession number text will not appear bold or underlined. In the final version of the electronic copy, the accession number text will be linked to the appropriate source in the NCBI databases, enabling readers to go directly to that source from the article.
For articles reporting the isolation of specific enzymes, the Results section should include a table listing each step of the procedure, and for that step the total protein, total enzyme units, and the calculated specific activity, percentage yield, and approximate purity. For proteins without catalytic activity, an analogous table should be included indicating for each step the total protein, the amount of specific protein of interest, and the calculated fold enrichment and percentage yield. Figures showing chromatographic elution profiles are appropriate if the separation being illustrated is complex and cannot adequately be described in the text.
Authors should indicate which aspects of the procedure have been optimized or are particularly critical to the success of the procedure.
Articles describing the isolation of specific proteins should report sufficient catalytic or physical properties of the purified protein to establish its identity. This information (Mr, subunits, pI, pH optimum, etc.) may best be presented in tabular form. Where isoforms are known or expected, the specific isoform purified should be identified. In general, only procedures leading to highly purified proteins will be considered for publication. Evidence of purity should therefore be provided and may include protein gels, immunological studies, or, for enzymes, comparison of the final specific activity with the specific activity obtained in previous studies.
Names of chemical or organic substances should follow the recommendations of the IUPAC-IUBMB Joint Combined Commission on Biochemical Nomenclature (JCBN).
Authors should draw attention to any particular chemical or biological hazards that may be involved in carrying out the experiments described. Any relevant safety precautions should be described; if an accepted code of practice has been followed, a reference to the relevant standards should be given. Since it is evident that the value of a purification or expression method is entirely dependent on the availability of all materials, sources of critical reagents and instruments must be clearly identified.
The journal accepts reports of studies involving recombinant DNA molecules, constructed in vitro and subsequently inserted into cells, with the understanding that the authors have adhered to appropriate NIH guidelines and/or other pertinent regulations. Procedures requiring special facilities or precautions should be identified and described in detail.
Figures. Number figures with Arabic numerals in order of appearance in the text. Type all legends consecutively on a separate sheet. All illustrations should be in finished form suitable for reproduction. On hard copies of the manuscript, identify all figures on the back lightly in soft pencil with the author(s) name(s) and figure number; indicate the TOP. On electronic copies, please indicate figure number in file name and on figure. Please visit our Web site at http://authors.elsevier.com/artwork for detailed instructions on preparing electronic artwork.
Photographs must be kept to a minimum. Magnification should be indicated by a scale where possible. Simple histograms should be avoided; a table or a paragraph in the text is preferred. Illustrations in color can be accepted only if the authors defray the cost.
Tables should be typed on separate pages, and numbered consecutively with Arabic numerals in order of mention in the text. Each table should have a short explanatory title.
References. Only articles that have been published or are in press should be included in the references. Unpublished results or personal communications should be cited as such within the text. Arrange the reference list in the order cited in the text and type double-spaced throughout. Full titles of the cited papers must be included. Cite references by Arabic numerals, on line, in brackets, for example, [4]. The names of journals should be abbreviated according to the Chemical Abstracts Service Source Index.
[1] R. Jayalakshmi, K. Sumathy, H. Balaram, Purification and characterization of recombinant Plasmodium falciparum adenylosuccinate synthetase expressed in Escherichia coli, Protein Express. Purif. 25 (2002) 66-72.
[2] R. Hesketh, The Oncogene FactsBook, Academic Press, San Diego, 1995.
[3] G.R. Mettam, L.B. Adams, How to prepare an electronic version of your article, in: B.S. Jones, R.Z. Smith (Eds.) Introduction to the Electronic Age, E-Publishing Inc., New York, 1999, pp. 281-304.
Proofs will be sent to the corresponding author. To avoid delay in publication, only necessary changes should be made, and proofs and manuscript should be returned promptly. Authors will be charged for alterations that exceed 10% of the total cost of composition.
Editorial Board
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- Editor-in-Chief:
- R.R. Burgess, University of Wisconsin, USA
- Executive Editors:
L.L. Bieber, Michigan State University
J.M. Cregg, Keck Graduate Institute of Applied Life Sciences
J.J. Dunn, Brookhaven Natinal Laboratory
O.W. Griffith, Medical College of Wisconsin
D.L. Jarvis, University of Wyoming
S.A. Lesley, Genomics Institute of the Novartis Research Foundation
- Editorial Board:
C. Batt, Cornell University
D. Black, National Institutes of Health
Z.F. Burton, Michigan State University
W.H. Campbell, Michigan Technological University
G.L. Cereghino, University of the Pacific
R.F. Colman, University of Delaware
A.J.L. Cooper, Weill Medical College of Cornell University
J.-M. Egly, INSERM U/184 Strasbourg
B.G. Fox, University of Wisconsin
D. Frank, Medical College of Wisconsin
H.J. George, Sigma-Aldrich Corporation
G. Georgiou, University of Texas
H.F. Gilbert, Baylor College of Medicine
E. Goldman, UNDNJ- New Jersey Medical School
F.P. Guengerich, Vanderbilt University School of Medicine
F. Hill, AVIDIS SA Biop鬺e Clermont-Limage
J. Kadonaga, University of California at San Deigo
A. Kruesch, Genomics Institute of the Novartis Research Foundation
R.E. Lanford, Southwest Foundation for Biomedical Research
R.V. Lewis, University of Wyoming
S.-H. Lin, University of Texas
D. Merkler, University of South Florida
S. Powers-Lee, Northeastern University
J. Preiss, Michigan State University
D.D. Randall, University of Missouri
F. Schroeder, Texas A&M University
R.K. Scopes, LaTrobe University
A.P. Seddon, Pfizer, Inc.
K. Severinov, Rutgers University
K. Soda, Kansai University
R.C. Stevens, Scripps Research Institute
F.W. Studier, Brookhaven Natinoal Laboratory
N. Taniguchi, Osaka University Medical School
D. Theilmann, Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada
B.F. Volkman, Medical College of Wisconsin
D.S. Waugh, National Cancer Institute
S. White, St. Jude Children's Research Hospital
M. Wilcheck, Weizmann Institute of Science
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