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期刊名称：CYTOTECHNOLOGY ISSN： 0920-9069 出版频率： Monthly 出版社： KLUWER ACADEMIC PUBL, VAN GODEWIJCKSTRAAT 30, DORDRECHT, NETHERLANDS, 3311 GZ   出版社网址： http://www.wkap.nl/ 期刊网址： http://www.kluweronline.com/issn/0920-9069 影响因子： 0.875(2008) 主题范畴： BIOTECHNOLOGY & APPLIED MICROBIOLOGY;    CELL BIOLOGY 期刊简介(About the journal)    投稿须知(Instructions to Authors)    编辑部信息(Editorial Board)    About the journal   Aims & Scope The exploitation of cell cultures in a biotechnological context is based upon research at all levels of academic and industrial input. Cytotechnology represents a central repository for information on both the infrastructure of cell technology and the applied use of cell cultures, thus providing a panoramic perspective of the many facets and disciplines needed to develop successful cell cultures. Cytotechnology is an international journal on new and novel developments, methods and techniques in fundamental cell culture research, and the applications of that research in human and vetinary medicine, toxicology and animal cell biotechnology. The journal publishes reviews, original papers, short communications and technical reports containing information on all forms of vertebrate and invertebrate in vitro cell cultures. Papers published in Cytotechnology include studies on: The derivation, genetic modification and characterisation of cell lines: genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology. Cell culture systems, processes, reactors, scale-up and industrial production. The application of cells in differentiation, cancer research, immunology, genetics, senescence, inflammatory and viral disease and other medical and veterinary investigation. The use of cell cultures as a substrate for bioassay, cytotoxicity and pharmacology measurement, biomedical applications, and in particular as a replacement for animal models. The importance of publishing factual reports on techniques, characterisation and standardisation of cell lines, and presenting updated information on international issues (e.g. import and export regulations, health and safety factors) is recognised by the inclusion of a special section entitled `Technical Reports'. Meeting reports and book reviews are also included.   Instructions to Authors   Kluwer Academic Publishers request the submission of manuscripts and figures in electronic form in addition to a hard-copy printout. The preferred storage medium for your electronic manuscript is a 3 1/2 inch diskette. Please label your diskette properly, giving exact details on the name(s) of the file(s), the operating system and software used. Always save your electronic manuscript in the word processor format that you use; conversions to other formats and versions tend to be imperfect. In general, use as few formatting codes as possible. For safety‘s sake, you should always retain a backup copy of your file(s). After acceptance, please make absolutely sure that you send the latest (i.e., revised) version of your manuscript, both as hard-copy printout and on diskette (submission in electronic form of the final version of your article is compulsory). Kluwer Academic Publishers prefer articles submitted in word processing packages such as MS Word, WordPerfect, etc. running under operating systems MS DOS, Windows and Apple Macintosh, or in the file format LaTeX. Articles submitted in other software programs can also be accepted. For submission in LaTeX, Kluwer Academic Publishers have developed a Kluwer LaTeX class file, which can be downloaded from: http://www.wkap.nl/authors/jrnlstylefiles/ Use of this class file is highly recommended. Do not use versions downloaded from other sites. Technical support is available through: texhelp@wkap.nl. If you are not familiar with TeX/LaTeX, the class file will be of no use to you. In that case, submit your article in a common word processor format. For the purpose of reviewing, articles for publication should be submitted as hard-copy printout (four-fold) and on diskette to: Journal Editorial Office Cytotechnology P.O. Box 990 3300 AZ Dordrecht The Netherlands Fax: +31 78 6576 555 Manuscript Presentation The journal‘s language is English. British English or American English spelling and terminology may be used, but either one should be followed consistently throughout the article. Cytotechnology welcomes general papers, review papers and short communications. General papers describing original research should not exceed ten pages of text (including references, tables and figures). Review papers should not exceed 25 pages of text (including references, tables and figures). Manuscripts should be printed or typewritten on A4 or US Letter bond paper, one side only, leaving adequate margins on all sides to allow reviewers‘ remarks. Please double-space all material, including notes and references. Quotations of more than 40 words should be set off clearly, either by indenting the left-hand margin or by using a smaller typeface. Use double quotation marks for direct quotations and single quotation marks for quotations within quotations and for words or phrases used in a special sense. Number the pages consecutively with on the first page: running head (shortened title) article type title subtitle author(s) affiliation(s) full address for correspondence, including telephone and fax number and e-mail address Abstract Please provide a short abstract of 100 to 250 words. The abstract should not contain any undefined abbreviations or unspecified references. Key Words Please provide 5 to 10 key words or short phrases in alphabetical order. Abbreviations Abbreviations and their explanations should be collected in a list. List only those which are not familiar and/or commonly used. Abbreviations and units SI units should be used, e.g.: mg, g, kg, km, m, cm, mm, ppm, cpm, l (litre), ml, s (second), min (minute), h (hour), mol, m-3, kg per ha or kg ha-1(the minus index form is always to be used in tables). Use mg l-1, not mg/l. If a non-standard abbreviation is to be used extensively, it should be defined under the heading ’Abbreviations‘. Figures and Tables Submission of electronic figures In addition to hard-copy printouts of figures, authors are requested to supply the electronic versions of figures in either Encapsulated PostScript (EPS) or TIFF format. Many other formats, e.g., Proprietary Formats, PiCT (Macintosh) and WMF (Windows), cannot be used and the hard copy will be scanned instead. Figures should be saved in separate files without their captions, which should be included with the text of the article. Files should be named according to DOS conventions, e.g., ’figure1.eps‘. For vector graphics, EPS is the preferred format. Lines should not be thinner than 0.25pts and in-fill patterns and screens should have a density of at least 10%. Font-related problems can be avoided by using standard fonts such as Times Roman and Helvetica. For bitmapped graphics, TIFF is the preferred format but EPS is also acceptable. The following resolutions are optimal: black-and-white line figures - 600 - 1200 dpi; line figures with some grey or coloured lines - 600 dpi; photographs - 300 dpi; screen dumps - leave as is. Higher resolutions will not improve output quality but will only increase file size, which may cause problems with printing; lower resolutions may compromise output quality. Please try to provide artwork that approximately fits within the typeset area of the journal. Especially screened originals, i.e. originals with grey areas, may suffer badly from reduction by more than 10-15%. AVOIDING PROBLEMS WITH EPS GRAPHICS Please always check whether the figures print correctly to a PostScript printer in a reasonable amount of time. If they do not, simplify your figures or use a different graphics program. If EPS export does not produce acceptable output, try to create an EPS file with the printer driver (see below). This option is unavailable with the Microsoft driver for Windows NT, so if you run Windows NT, get the Adobe driver from the Adobe site (www.adobe.com). If EPS export is not an option, e.g., because you rely on OLE and cannot create separate files for your graphics, it may help us if you simply provide a PostScript dump of the entire document. HOW TO SET UP FOR EPS AND POSTSCRIPT DUMPS UNDER WINDOWS Create a printer entry specifically for this purpose: install the printer ’Apple Laserwriter Plus‘ and specify ’FILE‘: as printer port. Each time you send something to the ’printer‘ you will be asked for a filename. This file will be the EPS file or PostScript dump that we can use. The EPS export option can be found under the PostScript tab. EPS export should be used only for single-page documents. For printing a document of several pages, select ’Optimise for portability‘ instead. The option ’Download header with each job‘ should be checked. Submission of hard-copy figures If no electronic versions of figures are available, submit only high-quality artwork that can be reproduced as is, i.e., without any part having to be redrawn or re-typeset. The letter size of any text in the figures must be large enough to allow for reduction. Photographs should be in black-and-white on glossy paper. If a figure contains colour, make absolutely clear whether it should be printed in black-and-white or in colour. Figures that are to be printed in black-and-white should not be submitted in colour. Authors will be charged for reproducing figures in colour. Each figure and table should be numbered and mentioned in the text. The approximate position of figures and tables should be indicated in the margin of the manuscript. On the reverse side of each figure, the name of the (first) author and the figure number should be written in pencil; the top of the figure should be clearly indicated. Figures and tables should be placed at the end of the manuscript following the Reference section. Each figure and table should be accompanied by an explanatory legend. The figure legends should be grouped and placed on a separate page. Figures are not returned to the author unless specifically requested. In tables, footnotes are preferable to long explanatory material in either the heading or body of the table. Such explanatory footnotes, identified by superscript letters, should be placed immediately below the table. Section Headings First-, second-, third-, and fourth-order headings should be clearly distinguishable but not numbered. Appendices Supplementary material should be collected in an Appendix and placed before the Notes and Reference sections. Notes Please use endnotes rather than footnotes. Notes should be indicated by consecutive superscript numbers in the text and listed at the end of the article before the References. A source reference note should be indicated by means of an asterisk after the title. This note should be placed at the bottom of the first page. Cross-Referencing In the text, a reference identified by means of an author‘s name should be followed by the date of the reference in parentheses and page number(s) where appropriate. When there are more than two authors, only the first author‘s name should be mentioned, followed by ’et al.‘. In the event that an author cited has had two or more works published during the same year, the reference, both in the text and in the reference list, should be identified by a lower case letter like ’a‘ and ’b‘ after the date to distinguish the works. Examples: Winograd (1986, p. 204) (Winograd 1986a, b) (Winograd 1986; Flores et al. 1988) (Bullen and Bennett 1990) Acknowledgements Acknowledgements of people, grants, funds, etc. should be placed in a separate section before the References. References References to books, journal articles, articles in collections and conference or workshop proceedings, and technical reports should be listed at the end of the article in alphabetical order. Articles in preparation or articles submitted for publication, unpublished observations, personal communications, etc. should not be included in the reference list but should only be mentioned in the article text (e.g., T. Moore, personal communication). References to books should include the author‘s name; year of publication; title; page numbers where appropriate; publisher; place of publication, in the order given in the example below. Hicks CR (1973) Fundamental Concepts in the Design of Experiments. Holt, Rhinehard and Winston, New York. References to articles in an edited collection should include the author‘s name; year of publication; article title; editor‘s name; title of collection; first and last page numbers; publisher; place of publication., in the order given in the example below. Smith EL, Austen BM, Blumenthal km & Nyc JF (1975) Glutamate dehydrogenases. In: Boyer PD (ed.) The Enzymes, Vol. 11 (pp. 293-367) Academic Press, New York. References to articles in periodicals should include the author‘s name; year of publication; article title; abbreviated title of periodical; volume number (issue number where appropriate); first and last page numbers, in the order given in the example below. Marquis CP, Low KS, Barford JP & Harbour C (1989) Agitation and aeration effects in suspension mammalian cell cultures. Cytotechnology 2: 163-170. References to technical reports or doctoral dissertations should include the author‘s name; year of publication; title of report or dissertation; institution; location of institution, in the order given in the example below. Leelavatcharamas V (1997) Growth, gamma interferon production and cell cycle in batch, continuous and perfusion culture of CHO cells. Ph. D. thesis, University of Birmingham, pp. 278. Proofs Proofs will be sent to the corresponding author by e-mail (if no e-mail address is available or appears to be out of order, proofs will be sent by regular mail). Your response, with or without corrections, should be sent within 72 hours. Please do not make any corrections to the PDF file. Minor corrections (+/- 10) should be sent as an e-mail attachment to: proofscorrection@wkap.nl. Always quote the four-letter journal code and article number and the PIPS No. from your proof in the subject field of your e-mail. Extensive corrections must be clearly marked on a printout of the PDF file and should be sent by first-class mail (airmail overseas). Offprints Fifty offprints of each article will be provided free of charge. Additional offprints (both hard copies and PDF files)can be ordered by means of an offprint order form supplied with the proofs. Page Charges and Colour Figures No page charges are levied on authors or their institutions. Colour figures are published at the author‘s expense only. Copyright Authors will be asked, upon acceptance of an article, to transfer copyright of the article to the Publisher. This will ensure the widest possible dissemination of information under copyright laws. Permissions It is the responsibility of the author to obtain written permission for a quotation from unpublished material, or for all quotations in excess of 250 words in one extract or 500 words in total from any work still in copyright, and for the reprinting of figures, tables or poems from unpublished or copyrighted material. Additional Information Additional information can be obtained from: Publishing Editor Cytotechnolgy Kluwer Academic Publishers Van Godewijckstraat 30 P.O. Box 17 3300 AA Dordrecht The Netherlands Phone: +31 78 6576123 Fax: +31 78 6576377 Homepage: http://www.kluweronline.com/issn/0920-9069   Editorial Board   Managing Editor: D.W. Barnes MDI Bio Lab, Salisbury Cove, ME, USA Otto-Wilhelm Merten Généthon II, Evry, France Sanetaka Shirahata Dept. of Genetic Resources Technology, Kyushu University, Fukuoka, Japan Advisory Editor: R.E. Spier University of Surrey, Guildford, UK J.B. Griffiths Porton, Salisbury, Wilts., UK Consulting Editors: N. Bols, University of Waterloo, Canada; J. Coco Martin, DSM Biologics, Groningen, The Netherlands; P. Collodi, Purdue University, West Lafayette, USA; A. Dickson, The University of Manchester, UK; O. Doblhoff-Dier, University of Agriculture, Vienna, Austria; S. Eridani, ITBA-CNR, Segrate, Italy; I. Freshney, University of Glasgow, UK; C. Harbour, University of Sydney, Australia; H. Hauser, Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany; J. Henderson, Coventry University, UK; M.E. Kaighn, Cell Applications Inc., San Diego, CA, USA; S. Kaminogawa, The University of Tokyo, Japan; R. Zwe-Ling Kong, National Taiwan Ocean University, Keelung, ROC; J.P. Mather, Raven Biotechnologies, San Carlo, CA, USA; W. McKeehan, Texas A&M University, Houston, USA; K. Nagai, Tokyo Institute of Technology, Yokohama, Japan; S.D. Park, Seoul National University, Korea; J. Pipas, University of Pittsburgh, PA, USA; L. de Ridder, University of Ghent, Belgium; A. Rizzino, University of Nebraska, Omaha, USA; D.K. Robinson, Merck Research Laboratories, Rahway, NJ, USA; G.N. Stacey, NIBSC, Hertfordshire, UK Journal Editorial Office Cytotechnology P.O. Box 990 3300 AZ Dordrecht The Netherlands Fax: +31 78 6576 555